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G Biosciences
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Image Search Results
Journal: BMC Biotechnology
Article Title: Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes
doi: 10.1186/s12896-021-00695-6
Figure Lengend Snippet: DNA extraction protocols tested on six individual adult, female T. circumcincta specimens per method in this study
Article Snippet:
Techniques: DNA Extraction, Extraction, Binding Assay, Cell Culture, DNA Purification
Journal: BMC Biotechnology
Article Title: Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes
doi: 10.1186/s12896-021-00695-6
Figure Lengend Snippet: Box-and-whisker plots of the DNA concentration (ng/μL) measured by ThermoScientific NanoDrop 2000™. 11 DNA extraction protocols were used, each with 6 individual adult, female T. circumcincta specimens. Boxes extend from the 25th to the 75th percentile of each group’s distribution of values; Whiskers above and below the box indicate the 10th and 90th percentiles. Median line __ ; Mean □ ; Outliers ♦; Dunn’s Multiple Comparison test significance between methods indicated by asterisk; *: 0.01 < p < 0.05; **: 0.001 < p < 0.01; ***: p < 0.001. Extraction method abbreviations: AccM (AccuPrep® Genomic DNA Extraction - Mammalian Tissue), AccW (AccuPrep® Genomic DNA Extraction Kit – Whole Blood, Buffy Coat and Cultured Cells), CheX (Chelex®100), CTAB (cetyl trimethyl ammonium bromide), EznF (E.Z.N.A.® Forensic DNA), IsoG (Isolate II Genomic DNA Kit), Schi ( Schistosoma sp. DNA Extraction Method), Schi-LE (Schi with larval exsheathment), SDS (sodium dodecyl sulphate), WizM (Wizard® Genomic DNA Purification Kit - Mouse Tail), WizP (Wizard® Genomic DNA Purification - Plant Tissue)
Article Snippet:
Techniques: Whisker Assay, Concentration Assay, DNA Extraction, Comparison, Extraction, Cell Culture, DNA Purification
Journal: BMC Biotechnology
Article Title: Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes
doi: 10.1186/s12896-021-00695-6
Figure Lengend Snippet: Box-and-whisker plots of the DNA concentration (ng/μL) measured by Invitrogen Qubit™ fluorometer. 11 DNA extraction protocols were used, each with 6 individual adult, female T. circumcincta specimens. Boxes extend from the 25th to the 75th percentile of each group’s distribution of values; Whiskers above and below the box indicate the 10th and 90th percentiles. Median line __ ; Mean □ ; Dunn’s Multiple Comparison test significance between methods indicated by asterisk; *: 0.01 < p < 0.05; **: 0.001 < p < 0.01. Extraction method abbreviations: AccM (AccuPrep® Genomic DNA Extraction - Mammalian Tissue), AccW (AccuPrep® Genomic DNA Extraction Kit – Whole Blood, Buffy Coat and Cultured Cells), CheX (Chelex®100), CTAB (cetyl trimethyl ammonium bromide), EznF (E.Z.N.A.® Forensic DNA), IsoG (Isolate II Genomic DNA Kit), Schi ( Schistosoma sp. DNA Extraction Method), Schi-LE (Schi with larval exsheathment), SDS (sodium dodecyl sulphate), WizM (Wizard® Genomic DNA Purification Kit - Mouse Tail), WizP (Wizard® Genomic DNA Purification - Plant Tissue)
Article Snippet:
Techniques: Whisker Assay, Concentration Assay, DNA Extraction, Comparison, Extraction, Cell Culture, DNA Purification
Journal: BMC Biotechnology
Article Title: Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes
doi: 10.1186/s12896-021-00695-6
Figure Lengend Snippet: Box-and-whisker plots of DNA quality measured by ThermoScientific NanoDrop 2000™ absorbency measurement 260 nm / 280 nm ratios. 11 DNA extraction protocols were used, each with 6 individual adult, female T. circumcincta specimens. Boxes extend from the 25th to the 75th percentile of each group’s distribution of values; Whiskers above and below the box indicate the 10th and 90th percentiles. The red line indicates the desired absorbency ratio of 1.8. Median line __ ; Mean □ ; Outliers ♦. Extraction method abbreviations: AccM (AccuPrep® Genomic DNA Extraction - Mammalian Tissue), AccW (AccuPrep® Genomic DNA Extraction Kit – Whole Blood, Buffy Coat and Cultured Cells), CheX (Chelex®100), CTAB (cetyl trimethyl ammonium bromide), EznF (E.Z.N.A.® Forensic DNA), IsoG (Isolate II Genomic DNA Kit), Schi ( Schistosoma sp. DNA Extraction Method), Schi-LE (Schi with larval exsheathment), SDS (sodium dodecyl sulphate), WizM (Wizard® Genomic DNA Purification Kit - Mouse Tail), WizP (Wizard® Genomic DNA Purification - Plant Tissue)
Article Snippet:
Techniques: Whisker Assay, DNA Extraction, Extraction, Cell Culture, DNA Purification
Journal: BMC Biotechnology
Article Title: Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes
doi: 10.1186/s12896-021-00695-6
Figure Lengend Snippet: Box-and-whisker plots of DNA quality measured by ThermoScientific NanoDrop 2000™ absorbency measurement 260 nm / 230 nm ratios. 11 DNA extraction protocols were used, each with 6 individual adult, female T. circumcincta specimens. Boxes extend from the 25th to the 75th percentile of each group’s distribution of values; Whiskers above and below the box indicate the 10th and 90th percentiles. The red line indicates the desired absorbency ratio of 2.0. Median line __ ; Mean □ ; Outliers ♦; Dunn’s Multiple Comparison test significance between methods indicated by asterisk; *: 0.01 < p < 0.05; **: 0.001 < p < 0.01; ***: 0.0001 < p < 0.001. Extraction method abbreviations: AccM (AccuPrep® Genomic DNA Extraction - Mammalian Tissue), AccW (AccuPrep® Genomic DNA Extraction Kit – Whole Blood, Buffy Coat and Cultured Cells), CheX (Chelex®100), CTAB (cetyl trimethyl ammonium bromide), EznF (E.Z.N.A.® Forensic DNA), IsoG (Isolate II Genomic DNA Kit), Schi ( Schistosoma sp. DNA Extraction Method), Schi-LE (Schi with larval exsheathment), SDS (sodium dodecyl sulphate), WizM (Wizard® Genomic DNA Purification Kit - Mouse Tail), WizP (Wizard® Genomic DNA Purification - Plant Tissue)
Article Snippet:
Techniques: Whisker Assay, DNA Extraction, Comparison, Extraction, Cell Culture, DNA Purification
Journal: BMC Biotechnology
Article Title: Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes
doi: 10.1186/s12896-021-00695-6
Figure Lengend Snippet: Agarose gels displaying amplicons produced by conventional PCR using primer pair ITS-2/NC2. Genomic DNA samples from single Teladorsagia circumcincta nematode specimens. Ladder indicating amplicon size (bp) (lanes 1, 14, 27). a Schi (lanes 2–7), Schi-LE (lanes 8–13), SDS (lanes 15–20), CheX (lanes 21–26), no-DNA control (lane 28), T. circumcincta ITS-2 gBlocks™ Gene Fragment (Integrated DNA Technologies) control (lane 29). b WizP (lanes 2–7), EznF (lanes 8–13), AccM (lanes 15–20), IsoG (lanes 21–26), no-DNA control (lane 28), T. circumcincta ITS-2 gBlocks™ Gene Fragment control (lane 29). C: AccW (lanes 2–7), WizM (lanes 8–13), CTAB (lanes 15–20), no-DNA control (lane 21), T. circumcincta ITS-2 gBlocks™ Gene Fragment control (lane 22). The specificity of individual amplicons produced was verified by direct sequencing
Article Snippet:
Techniques: Produced, Amplification, Control, Sequencing